anti a human chi3l1 Search Results


anti a human chi3l1  (R&D Systems)
93
R&D Systems anti a human chi3l1
Fig. 4. <t>CHI3L1</t> is auto-induced in cells with WT and mutant EGFR. NHBE, A549, H1975 and HCC827 cells were incubated in the presence and absence of rCHI3L1 and the levels of mRNA encoding CHI3L1 and <t>CHI3L1</t> <t>protein</t> accumulation were evaluated. The levels of mRNA encoding CHI3L1 were evaluated using qRT-PCR as illustrated in panel A. The levels of CHI3L1 protein accumulation can be seen in the Western blot evaluations and immunocytochemistry in panel B and C. The values in panel A represent the mean ± SEM of at least triplicate evaluations. The values in panels B and C are representative of at least 3 similar evaluations. (*p < 0.05 by t-test). Scale bar=25μm, it applies to every subpanel of Panel C.
Anti A Human Chi3l1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+a+human+chi3l1/Human+Chitinase+3-like+1%2FYKL-40+Antibody/pm39178575-239-8-11
Average 93 stars, based on 1 article reviews
anti a human chi3l1 - by Bioz Stars, 2026-09
93/100 stars
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β actin  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology β actin
Fig. 4. <t>CHI3L1</t> is auto-induced in cells with WT and mutant EGFR. NHBE, A549, H1975 and HCC827 cells were incubated in the presence and absence of rCHI3L1 and the levels of mRNA encoding CHI3L1 and <t>CHI3L1</t> <t>protein</t> accumulation were evaluated. The levels of mRNA encoding CHI3L1 were evaluated using qRT-PCR as illustrated in panel A. The levels of CHI3L1 protein accumulation can be seen in the Western blot evaluations and immunocytochemistry in panel B and C. The values in panel A represent the mean ± SEM of at least triplicate evaluations. The values in panels B and C are representative of at least 3 similar evaluations. (*p < 0.05 by t-test). Scale bar=25μm, it applies to every subpanel of Panel C.
β Actin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+a+human+chi3l1/%CE%B2-Actin+Antibody/pm39178575-239-23-24
Average 96 stars, based on 1 article reviews
β actin - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

Image Search Results


Fig. 4. CHI3L1 is auto-induced in cells with WT and mutant EGFR. NHBE, A549, H1975 and HCC827 cells were incubated in the presence and absence of rCHI3L1 and the levels of mRNA encoding CHI3L1 and CHI3L1 protein accumulation were evaluated. The levels of mRNA encoding CHI3L1 were evaluated using qRT-PCR as illustrated in panel A. The levels of CHI3L1 protein accumulation can be seen in the Western blot evaluations and immunocytochemistry in panel B and C. The values in panel A represent the mean ± SEM of at least triplicate evaluations. The values in panels B and C are representative of at least 3 similar evaluations. (*p < 0.05 by t-test). Scale bar=25μm, it applies to every subpanel of Panel C.

Journal: Translational oncology

Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.

doi: 10.1016/j.tranon.2024.102108

Figure Lengend Snippet: Fig. 4. CHI3L1 is auto-induced in cells with WT and mutant EGFR. NHBE, A549, H1975 and HCC827 cells were incubated in the presence and absence of rCHI3L1 and the levels of mRNA encoding CHI3L1 and CHI3L1 protein accumulation were evaluated. The levels of mRNA encoding CHI3L1 were evaluated using qRT-PCR as illustrated in panel A. The levels of CHI3L1 protein accumulation can be seen in the Western blot evaluations and immunocytochemistry in panel B and C. The values in panel A represent the mean ± SEM of at least triplicate evaluations. The values in panels B and C are representative of at least 3 similar evaluations. (*p < 0.05 by t-test). Scale bar=25μm, it applies to every subpanel of Panel C.

Article Snippet: The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.

Techniques: Mutagenesis, Incubation, Quantitative RT-PCR, Western Blot, Immunocytochemistry

Fig. 6. Effects of CHI3L1 inhibitors and TKI, alone and in combination, on the EGFR axis in vitro. HCC827 cells were incubated with rCHI3L1 and the effects of osimertinib (OSM) and FRG on the expression of CHI3L1, EGF and EGFR were evaluated by qRT-PCR (panels A–C). In panels D and E, H1975 cells were incubated with low doses of osimertinib (OSM) and FRG, alone or in combination, and the levels of mRNA encoding CHI3L1 and EGF were evaluated. In panel F, H1975 cells were incubated with osimertinib (OSM) and FRG antibody, alone or in combination, and the levels of apoptosis were evaluated by TUNEL staining. (*p < 0.05; **p < 0.01; *** p < 0.001, ****p < 0.0001 by ANOVA with multiple comparisons). ns, not significant.

Journal: Translational oncology

Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.

doi: 10.1016/j.tranon.2024.102108

Figure Lengend Snippet: Fig. 6. Effects of CHI3L1 inhibitors and TKI, alone and in combination, on the EGFR axis in vitro. HCC827 cells were incubated with rCHI3L1 and the effects of osimertinib (OSM) and FRG on the expression of CHI3L1, EGF and EGFR were evaluated by qRT-PCR (panels A–C). In panels D and E, H1975 cells were incubated with low doses of osimertinib (OSM) and FRG, alone or in combination, and the levels of mRNA encoding CHI3L1 and EGF were evaluated. In panel F, H1975 cells were incubated with osimertinib (OSM) and FRG antibody, alone or in combination, and the levels of apoptosis were evaluated by TUNEL staining. (*p < 0.05; **p < 0.01; *** p < 0.001, ****p < 0.0001 by ANOVA with multiple comparisons). ns, not significant.

Article Snippet: The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.

Techniques: In Vitro, Incubation, Expressing, Quantitative RT-PCR, TUNEL Assay, Staining

Fig. 8. Anti-CHI3L1 and TKI interact to inhibit tumor metastasis. WT mice were challenged with B16-F10 (B16) melanoma cells or control vehicle and treated with control IgG, FRG and or TKIs, alone or in combination. Melanoma lung metastasis was evaluated 2 weeks later. (A) Representative lungs from mice treated with control IgG, FRG and or gefitinib, alone or in combination. (B) The number of pleural melanoma colonies was quantitated in the lungs from the mice in panel A. (C) Representative lungs from mice treated with IgG, FRG and osimertinib, alone and in combination. (D) The number of pleural melanoma colonies was quantitated in the lungs from the mice in panel C. In panels B and D, each dot is representative of an individual animal. Panels A and C are representative of at least 3 evaluations. The values in panels B and D represent the mean ± SEM of the evaluations represented by the individual dots in the lungs from the experiments illustrated in panel A and C respectively. *P < 0.05. ***P < 0.001; ****P < 0.0001. by ANOVA with multiple comparisons). ns, not significant.

Journal: Translational oncology

Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.

doi: 10.1016/j.tranon.2024.102108

Figure Lengend Snippet: Fig. 8. Anti-CHI3L1 and TKI interact to inhibit tumor metastasis. WT mice were challenged with B16-F10 (B16) melanoma cells or control vehicle and treated with control IgG, FRG and or TKIs, alone or in combination. Melanoma lung metastasis was evaluated 2 weeks later. (A) Representative lungs from mice treated with control IgG, FRG and or gefitinib, alone or in combination. (B) The number of pleural melanoma colonies was quantitated in the lungs from the mice in panel A. (C) Representative lungs from mice treated with IgG, FRG and osimertinib, alone and in combination. (D) The number of pleural melanoma colonies was quantitated in the lungs from the mice in panel C. In panels B and D, each dot is representative of an individual animal. Panels A and C are representative of at least 3 evaluations. The values in panels B and D represent the mean ± SEM of the evaluations represented by the individual dots in the lungs from the experiments illustrated in panel A and C respectively. *P < 0.05. ***P < 0.001; ****P < 0.0001. by ANOVA with multiple comparisons). ns, not significant.

Article Snippet: The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.

Techniques: Control